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(I) qRT PCR assay was used to evaluate the expression level of genes that are located at different distances away from pFind1 insertion sites in pFind1-iPSC lines and control OKS-iPSC lines.
To evaluate the expression level of recombinant glucagon at a higher scale with controlled conditions, the WAVE bioreactor (GE Healthcare) was used at a scale up of 5 L (Figure 1A, lower lane).
To characterize the transcriptional landscape of human adult and fetal trabecular meshwork (TM), cornea, and ciliary body (CB) tissues, and to evaluate the expression level of candidate genes selected from genetic association studies of primary-open angle glaucoma, central corneal thickness, intraocular pressure, vertical cup to disc ratio, and optic nerve parameters.
In this approach, the ALP98 mouse mAb (directed against a linear epitope outside the mutated regions) [29] was used to evaluate the expression level of the mutated E1E2.
The SBC homemade human cDNA microarray (Shanghai, China) containing 15552 spots (including 768 controls and 14784 probes) was used to evaluate the expression level of 10379 known genes and 3022 ESTs.
To evaluate the expression level of neutral and autofluorescent bodies, a probe volume with xyz dimensions of 125 µm×125 µm×29 µm was defined at the mid-section of wild type or mutant worms.
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IHC was performed in all cases to evaluate the expression levels of the proliferation marker Ki-67, and to identify CD44-expressing cells, as potential CSCs.
The aim of this study was to evaluate the expression levels of DNA damage response (DDR) markers in potentially preneoplastic oral epithelial lesions (PPOELs).
The lysates were used to evaluate the expression levels of HIF1α by western blotting and frozen tumor sections were immediately processed for immunofluorescence analysis.
2×105 cells treated in the same way were seeded per well in 24 wells plates and lysed 24 h after to evaluate the expression levels of the BRET pairs.
In the current study, we used a previously validated microarray-based methodology [31] to evaluate the expression levels of 328 human miRNA in a series of extensively characterized ovarian surface epithelial tumors and normal fallopian tube samples.
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