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However, while assays exist to evaluate the division of precursor cells both in vivo (i.e. BrdU, Ki67, label retention) and in vitro (BrdU, cell counts, sphere forming assays) unraveling the contribution of stem cells from progenitor cells is difficult due to the lack of specific stem cell markers [31].
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Since the method does not provide the appropriate number of communities to split the network, the same authors [59] proposed to evaluate the divisions using the concept of modularity as the fitness function.
The extent to which the two relationships between global QOL and physical and mental health status were mediated by the life domains was evaluated as the division of the indirect-effects (mediated by the life domains) and the total effect (the sum of the direct- and indirect-effects for the associations between global QOL and physical and mental health status), expressed as a percentage [ 66].
Here, we used single-cell analyses to evaluate the cell division behavior of GSCs.
· Requires cost savings from this bill to be evaluated annually by the division of criminal justice and reported to the legislature and that some of the cost savings will be allocated to expand and enhance substance abuse treatment.
[ 27] In terms of unpaid work, Bird analyzed data from a national U.S. database to evaluate the relation between the division of household labour by married couples, and depression.
For the BrdU-uptake experiments, 10 microM BrdU (Sigma) was added from the beginning, but a toxic effect was seen with longer treatments than 5 days; to evaluate the % of cell division in spheroids of different days BrdU was added for the last 12 hours of incubation before fixation.
To evaluate the impact of the first division morphology on embryo development and IVF embryo transfer outcome.
After threshold determination, the stimulus response experiment was conducted to evaluate the MEP of the medial division of the DM of the affected and the nonaffected side of the patients and both sides in control subjects after TMS of the motor cortex on both hemispheres.
The homogeneity of the sequence alignment (as indicated by conserved stretches of residues and the absence of large gaps or inserts), a high bootstrap-value at the branching point that separates the orthologous cluster from the other sequences, and most importantly, a clear difference in conserved gene-context within the group were used to evaluate the necessity of such sub-division.
To evaluate the relationship of ERMES to mitochondrial division, wild-type or gem1Δ cells expressing Mdm12, Mdm34, or Mmm1-yEGFP and mito-DsRed were imaged using the spinning disc module of a Marianas SDC Real Time 3D Confocal-TIRF microscope (Intelligent Imaging Innovations Denver, CO, 3i) fit with a Yokogawa spinning disk head, a 100× 1.46 NA objective, and EMCCD camera.
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