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To evaluate the differentiation of MSCs, osteoblastic differentiation markers, such as alkaline phosphatase (ALP) activity and mineralization, were investigated.
An ALP assay has been utilized in numerous in vitro studies to evaluate the differentiation of MC3T3-E1 cells [24, 25].
The purpose of this study was to evaluate the differentiation of neurons from NT2 cells, a model NPC cell line, following hTERT transduction.
Reverse transcription-polymerase chain reaction (RT-PCR) analysis was used to evaluate the differentiation of bWJ-MSCs into neuron-like cells.
In this study, we tried to implant BMSC transvenously to a bleomycin-induced lung injury model, and evaluate the differentiation of BMSC.
To evaluate the differentiation of SMG cells, embryonic stem cell markers and salivary gland markers were used for qPCR and for immunohistological analysis.
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The data-set composition plays a central role when evaluating the differentiation of actives and inactives by a fingerprint [12].
Though DAT ligands are reportedly superior for detecting decreases in DA neurons [33 35], [18F]FDOPA enables examination of dopaminergic neuron functions including transport, decarboxylation, and vesicular uptake, which is useful for assessing the neuroprotective effects of therapeutic agents, as well as evaluating the differentiation of grafted cells in living brains.
Next, we evaluated the differentiation of IhNSC-Ps into specific neuronal and glial phenotypes following transplantation into the ischemic environment by analyzing the colocalization of selective markers for neurons, astroglial and oligodendroglial cells with the anti-human specific antibody anti-huN.
All our results are summarised in Figure 5. First we evaluated the differentiation of normal urothelium and that of low and high grade carcinomas.
In evaluating the differentiation of BM-MSCs to SLCs, immunocytochemical staining revealed that BM-MSCs to SLCs were positive for S-100 (82.3 ± 5.8%).
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