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To evaluate the differentiation, in brief, cells were fixed with buffered formalin (10%) in PBS for 10 mins at room temperature and stained with oil red-O for 1 h to assess lipid formation for adipogenesis or naphthol-AS-BI phosphate and fast red for 1 h to assess alkaline phosphatase activity for osteogenesis.
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To evaluate the differentiation program in our Esrp DKO mice we performed indirect fluorescent immunohistochemistry for epidermal differentiation markers.
For tumor grading, Edmondson and Steiner grading system was used to evaluate the differentiation status of HCC in this study [ 17].
To further evaluate the differentiation potential of ASCs in the fibrin hydrogel we quantified the glycerol release of the cells within the fibrin clots in the supernatant.
An ALP assay has been utilized in numerous in vitro studies to evaluate the differentiation of MC3T3-E1 cells [24, 25].
In our approach, we evaluated the differentiation potential of AM in toto with its sessile stem cells as alternative to conventional approaches requiring cell isolation and combination with biomaterials.
Though DAT ligands are reportedly superior for detecting decreases in DA neurons [33 35], [18F]FDOPA enables examination of dopaminergic neuron functions including transport, decarboxylation, and vesicular uptake, which is useful for assessing the neuroprotective effects of therapeutic agents, as well as evaluating the differentiation of grafted cells in living brains.
We then evaluated the differentiation and stem-associated markers in normal epidermis through a double staining with survivin.
In evaluating the differentiation of BM-MSCs to SLCs, immunocytochemical staining revealed that BM-MSCs to SLCs were positive for S-100 (82.3 ± 5.8%).
Polymorphism at the common set of 676 merged nuclear contigs was used to evaluate the genetic differentiation in pairwise comparisons between species.
To evaluate the neural differentiation potential of cells obtained in our protocol, the rosettes were mechanically cut and plated on ornithin/human laminin coated chamber slides in GRM/bFGF medium.
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