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The aim of this study was to develop an ELISA capable of detecting a fragment of type V collagen generated by MMP-2/9 and to evaluate the assay as biomarker for ankylosing spondylitis (AS).
One hundred and fifty-four positive and 15 negative blood culture samples were collected to evaluate the assay performance.
However, to date there have been few reports that objectively evaluate the assay completion rate, call rate and accuracy of APEX.
The PCR fragments of the M, H5 and N1 genes of H5N1 virus (A/Vietnam/1203/04) were hybridized separately or simultaneously to further evaluate the assay specificity (Fig. 2C).
Because the intent was to evaluate the assay in the context of routine screening and the centres used were experienced at screening, no attempt at study wide control of colonoscopy or pathology procedures or quality was made.
In this study, we genotyped 400 non-HapMap Japanese samples using the SNP Array 6.0 platform in order to evaluate the number of SNPs available for GWAS in the Japanese population, to examine an appropriate approach for acquiring accurate genotype calls using the Birdseed genotype calling algorithm, and to evaluate the assay criteria for preventing low-quality genotyping data.
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We evaluated the assay using 49 taeniid egg-positive samples collected from dogs in Zambia.
A study performed by Illumina evaluated the assay reproducibility and various technical aspects [2] and a more recent paper reported on an optimized protocol for sample preparation [15].
MM and EWP provided laboratory methodology and expertise in evaluating the assay results.
We earlier evaluated nasopharyngeal secretions (swabs) and now made a small pilot evaluating the assay performance on serum DNA from NPC and controls.
The coverage of the RT-LAMP primers was validated by evaluating the assay using viral RNA extracted from the different DENV strains, as described above.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com