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Afterwards, the activated samples with better cleaning performance in terms of resulting NW quality were carefully analyzed by cyclic voltammetry to evaluate the activation of the gold catalyst layer, using it as an indicator of the seed-layer quality for subsequent NW growth.
To further evaluate the activation of Hsf-1 by MGO-induced stress, we examined the ability of Hsf-1 to bind heat-shock elements (HSE) on DNA and activate transcription of the genes under the control of this conserved sequence.
We used Western blotting analysis to evaluate the activation of ATM in H1299 cells in response to ectopic activation of NPRL2 in the presence or absence of cisplatin (Figure 2A).
However, it is difficult to evaluate the activation of meridians.
We used the cell surface marker fibroblast activation protein (FAP) to evaluate the activation of fibroblasts.
Inflammatory cytokines elicited by the microbicide candidates are frequently used as biomarkers to evaluate the activation of topical mucosal immunity.
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To determine whether B cells contribute to CD4 T cell activation in the context of active disease, we evaluated the activation of both CD4 T cells and B cells by Leishmania antigens in PBMC cultures.
Using an antibody that specifically recognizes an epitope overlapping the nuclear localization signal of the p65 subunit and, thus, selectively binding to the activated form of p65 (indicated as p65*), we evaluated the activation of NF-κB in single and double transgenic mice at the onset stage by Western blot.
In the same cell populations, we studied Src activity by the in vitro kinase assay evaluating the activation of Hck, a member of Src kinases.
As Shc can also be recruited by IGF-1R activatevate downstream SH2-domain containing proteins, we also evaluated the activation of this alternative pathway.
Having established that BV activates the PI3K-Akt signaling axis [1], we next evaluated the activation of pAkt and pS6 (downstream of mTOR) in response to BV in RAW 264.7 macrophages.
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