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# 218073) in an ABI7900HT Real-Time PCR system using (Applied Biosystems, Foster City, CA) with 95 °C for 15 min followed by 40 cycles (94 °C for 15 s, 55 °C for 30 s and 72 °C for 30 s) followed by a melting curve step to evaluate specificity of amplification.
10 healthy subjects were examined to evaluate specificity.
Therefore, we will evaluate specificity of fecal calprotectin in children presenting in primary care.
PCR products were sequenced to evaluate specificity of the primer pairs.
Melting curves from 60°C to 99°C were assessed to evaluate specificity.
To evaluate specificity of gingival recession, a minor diagnostic criterion for vEDSin the Villefranche classification.
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To evaluate specificities of the welcoming moment in intensive care and assess how caregivers try and overcome difficulties.
We evaluated specificity and also compared the sensitivity and kinetics of the bead-based ALISSA to those of the bead-free conversion of the reporter peptide.
We designated the presence of S. pneumoniae or other bacteria in the control children who had no evidence of respiratory tract infection as "false positives" and evaluated specificity accordingly.
Western blotting was performed as an independent means of evaluating specificity of NN-Ab06 (11 ).
The validation strategy concentrated on evaluating specificity, dynamic range, reproducibility and sample stability.
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