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To evaluate of differentiation, control and treated cells were removed from the Petri dishes, pelleted by centrifugation, and resuspended in 1 ml fresh medium.
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Mann-Whitney U-tests were used to evaluate significance of differentiation of any two patient groups by various miRNA pairs.
Briefly, Mann-Whitney U-tests were used to evaluate significance of differentiation of any two patient groups by various miRNA pairs, and Spearman's rank correlation coefficient was calculated to estimate associations between various miRNAs.
To evaluate differentiation of fibroblasts into myofibroblasts, the expression of α-SMA was assessed in cells under culture conditions in the presence of FBS and HS.
The aim of this study was to evaluate osteogenic differentiation of MSC seeded on a collagen scaffold.
The purpose of this study was to evaluate the differentiation of neurons from NT2 cells, a model NPC cell line, following hTERT transduction.
The viability was compared, and immunohistochemistry was used to evaluate differentiation of the skin constructs using different scaffold materials.
We used NBT reduction to evaluate differentiation of AML cells.
In order to evaluate differentiation of monolayers, we measured transepithelial electrical resistance (Millicell-ERS, Millipore Corp., Bedford, MA) and the electrical resistance was approximately 500 Ω/cm, indicating that the cells achieved tight junction formation.
To evaluate the differentiation of MSCs, osteoblastic differentiation markers, such as alkaline phosphatase (ALP) activity and mineralization, were investigated.
An ALP assay has been utilized in numerous in vitro studies to evaluate the differentiation of MC3T3-E1 cells [24, 25].
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