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The virus was used to transfect cultured mouse endothelial cells (SVEC) and rat neonatal cardiac myocytes to evaluate expression of the protein by Western blotting.
In addition, they conducted an immunological and histological chemical study to evaluate expression of the MMP7 protein, whose gene was the one most overexpressed in normal tissue.
We also evaluate expression of the highly upregulated cell adhesion and angiogenic genes because they have been subject to previous studies in squamate reptiles (Biazik et al. 2007, 2008, 2010; Murphy, Belov, et al. 2010; Wu et al. 2011) and therefore provide a rare opportunity to assess the diversity of molecular mechanisms involved in reptilian pregnancy.
To evaluate expression of the PTPN22 splice forms, we measured the expression levels of PTPN22-long isoforms and the short isoform PTPN22_v4 (LYP2; Figure 1), relative to ZNF592 expression in PBMCs of healthy individuals and RA patients (cohort I) after 6 h of cultivation of cells without stimulation.
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ELISA was performed to evaluate expressions of the DAG and IP3 as previously described [ 26].
To disclose the molecular basis underlying G1-S phase transition by SSTR1, we evaluated expression of the key regulatory factors that control G1-S checkpoint.
To ascertain that this phenotype was leukocyte-specific, we evaluated expression of the pik3cd gene (which codes for p110δ) in non-hematopoietic cells at the maternal-fetal interface.
Since MMP2 displayed oxygen-dependent generation of its active form, we evaluated expression of the two essential members of the complex responsible for activation of pro-MMP2 at the cell surface, tissue inhibitor of MMP 2 (TIMP2) and MMP14.
The Genevestigator database [ 37] was used to evaluate expression differences of the selected genes.
Evaluate expression of MMP-13 during the course of two models experimentally induced periodontal disease in rats.
We next evaluated expression and secretion of the T3sVHHs by Western blot (WB).
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