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3- 4,5-dimethylthiazol-2yl- -2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate cell viability.
The methylthiazol tetrazolium (MTT) assay, a cell apoptosis study and the flow cytometry were used to evaluate cell viability of mouse fibroblast L929.
After the hyperthermia test, the cells were incubated for 24 h prior to undergoing MTT assay to evaluate cell viability.
[3- 4,5-Dimethylthiazol-2-yl -2,5-diphenyl tetrazolium bromide] (MTT) assay was used to evaluate cell viability, measuring mitochondrial dehydrogenase activity as previously described [3- 4,5-Dimethylthiazol-2-yl -2,5-diphenyl
The remaining cell solutions in cultured 96-well plate were used to evaluate cell viability by 3- 4,5-dimethylthiazole-2-yl -2,5-diphenyl tetrazolium bromide (MTT) assay.
The MTT [(4,5-dimethylthiazoyl-2-yl -2,5-diphenyltetrazolium bromide)] reduction assay, based on the conversion of tetrazolium salts to formazan crystals, was used to evaluate cell viability on the basis of mitochondrial activity, following the method described by Mosmann [4,5-dimethylthiazoyl-2-yl -2,5-diphenyltetrazolium
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We evaluated cell viability and functions, and also monitored the oxygen consumption.
We evaluated cell viability at different times after transfection by performing a trypan blue exclusion test.
The capacity of 5-FU to induce cell death is abrogated by co-treatment with KGF, whereas in KGFR silenced cells 5-FU efficiently induces cell death even combined to KGF, as determined by evaluating cell viability.
To rule out a possible cell type or species-specific effect for E- or A-Bax mutants, similar studies were performed in human H157 epithelial lung cancer cells using luciferase assay for evaluating cell viability as described [38] [39].
Cortical and cerebellar neurons were treated with or without MG132 for 8 hours (long enough to induce accumulation of SP-PrP in cortical cells), then incubated for 16 hours with or without staurosporine, before evaluating cell viability by MTT assay.
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