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To evaluate cell proliferation, Ki-67, a marker of proliferating cells, was immunolabeled in transverse frozen sections of rat brain, at 7 days after MCAO (Anti-Ki-67, 1 : 300, Abcam).
Fibroblast culture was performed to evaluate cell proliferation on the copolymers films.
Finally, human fibroblasts were cultured in the presence of nanoparticles to evaluate cell proliferation and cytotoxicity.
MTT (3- 4,5-dimethylthiazol-2-Yl -2,5-diphenyltetrazolium bromide) assay is the most commonly used assay to evaluate cell proliferation and viability [21].
WST-8 based Colorimetric Assay Cell Counting kit 8 (CCK-8) in vitro and xenograft transplantation model in nude mice was used to evaluate cell proliferation and growth respectively.
Study Design: Immunohistochemical methods were used to evaluate cell proliferation, vascular density, and α-smooth muscle actin expression by stromal cells in a group of 9 age-matched intrauterine growth restricted and control placentas at 25 to 41 weeks of gestation.
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We evaluated gliogenesis subsequent to H/I using immunofluorescence methods as well as by evaluating cell proliferation within the SVZ and by fate mapping SVZ cells using replication deficient retroviruses.
11C-4DST might be superior to 18F-FLT in evaluating cell proliferation and treatment response and predicting prognosis as 11C-4DST uptake represents the whole DNA synthesis process.
We hypothesized that 11C-4DST isuperioror to 18F-FLT in evaluating cell proliferation as 11C-4DST uptake represents the whole DNA synthesis process.
A fluorinated thymidine analogue, 3′-deoxy-3′-[18F]fluorothymidine (18F-FLT) (Figure 1A), has emerged as a promising positron emission tomography (PET) tracer for evaluating cell proliferation in various malignant brain tumors [1-4].
Moreover, the biocompatibility of the plasma-treated films was studied with MG-63 human osteoblast-like cells, evaluating cell proliferation, viability and morphology at 1, 3 and 7 days.
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