E9 chick DRG were seeded on the electrospun scaffolds to evaluate cell attachment and neurite extension.
A convenient approach to evaluate cell apoptosis is AO/EB staining.
The attached cells were quantified to evaluate cell adhesion on CNH-Col and collagen substrates.
Finally, human fibroblasts were cultured in the presence of nanoparticles to evaluate cell proliferation and cytotoxicity.
Fibroblast culture was performed to evaluate cell proliferation on the copolymers films.
To evaluate cell adhesion and cytoskeleton reorganization, immunostaining specific to vinculin and actin filaments was performed on nanodot arrays (Figure4).
In this study, E9 chick DRG were seeded on the electrospun scaffolds to evaluate cell attachment and neurite extension.
After the hyperthermia test, the cells were incubated for 24 h prior to undergoing MTT assay to evaluate cell viability.
The TUNEL assay was used to evaluate cell apoptosis according to the manufacture's instruction (Calbiochem, Darmstadt, Germany).
To evaluate cell growth, culture pre-grown overnight was diluted 1 500, and doxycycline was added if necessary.
Mouse fibroblasts (L929) were seeded onto the non-irradiated and irradiated surfaces to evaluate cell attachment capabilities.
