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Materials for the high gf verbal blocks were adapted with permission from a standard letter-based problem-solving task, Letter Sets from the Educational Testing Service (ETS) kit of factor-referenced tests (Ekstrom et al. 1976).
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Dye terminator cycle sequencing using the DYEnamic ET kit (Amersham Biosciences) was used to confirm the complete cDNA sequences for all FP variants and fusion constructs.
Sequencing reactions were performed with the DYEnamic ET terminator kit.
Sequencing reactions were performed with DYEnamic ET Terminator Kit (GE Healthcare Life sciences, UK).
Sequencing reactions were performed using DYEnamic ET Terminator Kit (GE Healthcare Life sciences, UK).
PCR products were, prior to sequencing, purified with ExoSAP-IT reagent (Amersham Biosciences) followed by direct sequencing in forward and reverse directions using the DYEnamic™ ET DyeTerminator Kit (Amersham Biosciences).
The resulting cassette was cloned into the EcoRV site of plasmid pBS (pBS-PTEF crtS-Tact, Table 2) and was sequenced on both strands using a DYEnamic ET Terminator Kit (Amersham Bioscience) in an ABI 3100 Avant genetic analyzer.
An aliquot of each amplification product was analyzed on a 1% agarose gel before another 1 μl aliquot was submitted to DNA sequencing using standard protocols of the DYEnamic™ ET Terminator kit (Amersham Biosciences).
Cutaneous melanoma is characterised by activating driver mutations in genes such as BRAF (Davies et al, 2002), NRAS (Ball et al, 1994), and KIT (Curtin et al, 2006), and recurrent losses of specific tumour suppressors such as CDKN2A and PTEN (Curtin et al, 2005).
Sequencing was performed on MegaBace 500 capillary DNA sequencer (Amersham Biosciences) using DYEnamic ET Terminator Kits with Thermo Sequenase™ II DNA Polymerase (Amersham Biosciences).
Sequencing was performed on a MegaBace 500 capillary DNA sequencer (Amersham Biosciences) using DYEnamic ET Terminator Kits with Thermo Sequenase II DNA Polymerase (Amersham Biosciences).
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