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Elution was carried out using an n-hexane: octane ethyl mixture (60 40% v/v).
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The ethyl cellulose mixture with ethanol was agitated for enough time for the mixture to completely fuse in the ethanol.
The P(BnMA) grafted MNs were suspended in a variety of solvents namely toluene, acetone, tetrahydrofuran, dichloromethane, and ethyl acetate/water mixture.
The ethyl acetate mixture was washed with 5 % HCl (30 mL × 3) and saturated NaCl (30 mL × 3).
It was purified by recrystallization from ethyl acetate/hexane mixture (1 1 vol/vol).
The optimal conditions were 1 g of seaweed sample, 4 g of anhydrous sodium sulphate as dispersant, 3.6 g of Florisil and 0.4 g of GCB and an elution volume of 14 mL of a hexane:ethyl acetate mixture containing 40% ethyl acetate.
The model was adequately validated by simulation of the removal of a 1 1 (w/w) ethyl acetate/toluene mixture in a peat biofilter operating under similar operating conditions than in the single pollutant biofiltration.
Finally, aiming at examining the model capabilities to describe multicomponent systems, VLE measurements involving two alcohols and the fatty acid ethyl ester mixture obtained from non-edible vegetable oil have been carried out showing the good performance of the predictive models.
The pellet was washed three times using 1 1 (v/v) ethanol:ethyl acetate mixture (1 mL).
Extraction was performed using an ethyl acetate/isopropanol mixture (85 : 15, v/v).
The residue was purified by flash column chromatography on silica gel with an ethyl acetate/heptane mixture (10 90 to 30:70).
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