Exact(16)
In view of the broad range of GC contents in the P. infestans CDS analyzed (ESTs), it is clearly difficult to separate sequences belonging to host and pathogen merely by GC content.
Since this dataset is composed of public ESTs, it contains a large amount of redundancy.
Considering only those contigs representing 20 or more ESTs, it is found that most of the genes are usually represented in multiple libraries.
While annotation from these resources can be accessed through web-based homology searches, for many laboratory collections of ESTs it is difficult to use existing tools to achieve a systems-level view of gene functions and relationships.
Because the genome sequences of relevant host species are not available or available but not well matched with their ESTs, it remains to be established whether they represent authentic expressed endogenous viral sequences.
Since this method does not rely on ESTs, it avoids the problems of sampling errors and noise inherent to ESTs, and can therefore provide robust assessment on the absolute number of conserved alternatively spliced exons in the human genome.
Similar(44)
Update 2 @ 1 50 pm EST:It might be coming back…we're getting a flood of emails now.
But when it did start at around 6PM EST, it knocked New York on its ass.
20.13 EST It's time for the "Society" award - Non-profit/NGOs.
The call took place at 2.30 EST, it lasted about 15 minutes and took place through an interpreter.
As theology it goes back further still, all the way to the third century and the magnificent paradox of Tertullian: Certum est quia impossibile est, it is certain because it is impossible.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com