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This observation needs further validation in a larger prospective study with well-defined BNP estimation time points, but it does suggest that serial BNP measurement may be helpful in identifying infants at high and low risk for mortality; and that treatment, including the intensity of surveillance and the use of aggressive pharmacologic and interventional therapy, may be adjusted accordingly.
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Fortunately, in the case of time course data, regression based methods can borrow information for error estimation across time points [ 6, 7].
For PK parameter estimation, the actual time points of sample collection were considered.
We define several biologically relevant metrics, which are scalar functions of the parameter and state estimations after incorporating estimated candidate time point measurements.
From the discussion above we thus motivate parameter estimation in three regions, rather than one set of parameter estimates for all time points.
An adaptive trial design enables sample size re-estimation at interim analyses time points during the trial [ 29].
However, we found that the parameter estimation for a combination of time points can be directly obtained by intersecting the individual estimated parameter spaces.
For chlorophyll estimation, tissues harvested at different time points were ground in 80% chilled acetone.
Thus, no meaningful estimation of the time point for the metabolic switch was achieved.
Kidney function was assessed by glomerular filtration rate (GFR) estimation at the time point of kidney biopsy and after consecutive immunosuppressive therapy.
The second model (with interaction) confirmed that there was an interaction between time and treatment (p = 0.03) and differences between the study groups in pain estimations occurred at the following time points: 120 minutes (MA pain scores lower than EA), 270 minutes (MA pain scores lower than EA), and 360 minutes (SC pain score lower than MA).
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