NMG and PV did lignin estimation of the mutants.
We have added a figure that schematically shows an estimation of the relative mutant frequencies.
A key finding of the present work is that the double-mutant fitness matrix alone carries enough information for accurate estimation of the single-mutant fitness values and for prediction of functional relationships among the genes.
Accordingly, the double-mutant fitness matrix alone should carry enough information for accurate estimation of the single-mutant fitness values, which typically are not measured in the large-scale interaction experiments, but could be estimated computationally.
However, it was soon found out that the built-in sequential procedure, which considers such increasingly larger subsets of mutation pairs in W that best fit the multiplicative model to improve the estimation of the single-mutant fitness vector w, was computationally infeasible when faced with the massive data matrices originating from the high-throughput genetic interaction experiments.
More specifically, the estimation of the single-mutant fitness effects is based on sub-sequent calculation of the p and q-quantile points for the rows and columns of the double-mutant fitness matrix W, respectively, and then arranging these quantiles in the estimated array and query vectors x and y.
We note, however, that we have not demonstrated that the assembly of wild-type and mutant PA63 monomers occurs in an even ratio and in a purely random manner, so our estimation of the number of mutant monomers required to exert dominance in a hetero-oligomer may be inexact.
However, electrophysiological recordings have not yet been combined with biochemistry techniques that enable quantitative estimation of the localization of mutant channel proteins at the plasma membrane.
Similar to the dSLAM approach, the estimation of the individual double mutants' relative growth rates was performed by using bar-code DNA microarrays.
An additional advantage of the LI-COR system, is that the use of different dyes for each primer allows a precise estimation for the location of the mutant.
