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We validated our estimation of the evolutionary rate by grouping strains according to the estimated date of their pair wise last common ancestor.
Xiang, Y; Ding, J; Tarokh, V, Estimation of the evolutionary spectra with application to stationarity test, Ieee Transactions on Signal Processing, vol 67 no. 5 (2019), pp. 1353-1365 [abs1109/TSP.2018.2890369] [abs].
Relative rate test which was used for the estimation of the evolutionary rate among lineages was performed by the pair-wise comparisons of synonymous substitutions (Ks) and non-synonymous substitutions (Ka) for nucleotide and amino acid variations for protein sequences; the node A sequence was used as an outgroup.
Gene trees summarize our current estimation of the evolutionary relationships among the LysRS sequences.
The reconstruction of phylogenetic trees from biological sequences relies on estimation of the evolutionary distance between the sequences of interest.
In most eukaryotes, the most evolutionarily conserved genes are the rRNA genes; comparison of their sequences allows estimation of the evolutionary distances at intergeneric and higher taxonomic levels.
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Annotation errors could lead to an over-estimation of the evolutionary rate in duplicated genes [ 56].
Estimations of the evolutionary distances by several substitution models (JC69, F84 and HKY85) are saturated for half of our data set and, therefore, were not retained for analysis.
The inspection of posterior distributions and the estimation of the relevant evolutionary parameters were done with TRACER version 1.5 (tree.bio.ed.ac.uk/software/tracer).ac.uk/software/tracer
Roundup uses BLAST for all-in-all comparisons, ClustalW for constructing MSA, PAML for the ML estimation of the of evolutionary distance and Python scripts that intermediate several processes, for example, format conversion, etc.
We note that in our study, we did not consider the estimation of the macro evolutionary parameters, and did not use unresolved trees, that can be used in Medusa to account uncertainties in the phylogeny.
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