To estimate cell surface fluctuations, the measurement time of the I D curve was checked against a known standard [16, 18, 19]. Figure 2b shows the representative I D curves for L929 cells with different CPFA's.
To estimate cell surface expression of receptors carrying an N-terminal HA-tag, we used an indirect cellular ELISA [57].
Using an in vitro model of primary hepatocyte culture that stabilizes PHH function, is oxygen-responsive, and infectible with liver-stage malaria, we applied a mathematical framework to estimate cell surface oxygen tensions under a variety of experimental manipulations.
Subsequently, cells were stimulated with TG (solid lines) or left unstimulated (hashed lines) and incubated with BTX to estimate cell surface Orai1 as described in Figure 3 figure supplement 2. BTX binding to Orai1-YFP expressing cells was used as a control (gray line).
We chose a biomass estimate – cell surface area – to determine exposure to cyanobacteria, rather than the traditional reporting method of cell counts per unit volume of water [[ 9] (Chapter 3)].
To estimate the cell surface oxygen tensions in MPCCs, the transport and consumption of oxygen was modeled as a one-dimensional reaction-diffusion system, as described previously (Yarmush et al., 1992).
Using this model system and a mathematical framework to estimate the cell surface oxygen partial pressures (pO2) under a variety of experimental manipulations, we show that oxygen has a profound impact on the Plasmodium liver stage.
Absorption properties of extracellular matrix (ECM) were investigated for carbon negative-ion-implanted polymers of polystyrene (PS), polydimethylsiloxane (PDMS) and poly-lactic acid (PLA) to estimate cell attachment properties of the modified surface without cell culture.
Stable expression of Orai1-BBS-YFP followed by exogenous application of labeled BTX provided an estimate of cell surface Orai1 levels.
