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Due to the fact that dwarf males and shoots within colonies are not independent samples, genetic diversity estimates on population level were based on a mean of 1000 permutations with one random individual from each colony sample (dwarf male or shoot).
HIV prevalence estimates for the MSM and IDU-FSW subpopulations fall within the estimated range of prevalence given in [ 51], where the upper and lower estimates from the data were calculated using low estimates for the number infected with high estimates on population size, and high estimates for the number infected with low estimates of population size, respectively.
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Karachi's current population (estimated on population census of 1998) is approximately 15 million [ 29].
First, our models are based on deterministic estimates of population growth, which typically over-estimate true stochastic growth rates [19].
The screening method relies on accurate estimates of population vaccine coverage (25 ).
It should be noted that time estimates based on population genetics also depend on molecular clock calibration, as described above, and also require a number of assumptions to be made about the population demography.
Multiple factors (e.g., mutation rates, effective vs. census population sizes, and percentage of immigrants successfully breeding) made it difficult to convert estimates based on population genetics to absolute numbers of immigrant individuals.
Estimates based on population studies were favoured, but in some particular cases where such studies were not available (e.g. Coffea canephora, Macadamia integrifolia), values determined from preliminary screen panels had to be used.
If possible, some uncertainty estimates on the population at risk rather than a single point estimate would be helpful.
Moreover, while estimates on general population are essential to better illustrate drug trends, it is also important to identify and assess consumption prevalence among key groups and target populations.
Rates of use in each SSD were then estimated based on population size.
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