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31 Estimates of variation and consistency are based on back transformations to differences on a 10 cm visual analogue scale.
Estimates of variation and genealogical relationships from the displacement (D) loop region (299 bp) are contradicted by the complete mitochondrial genome, as well as the protein coding fraction of the mitochondrial genome.
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Using pessimistic estimates of variation, heritability, and selection intensity, Nilsson and Pelger came to the conclusion that it would take 364,000 generations for a fish eye to evolve.
While it is not possible to measure selective constraint on individual proteins directly using this approach, it has been demonstrated that when a group of genes are measured together, estimates of variation are robust and in good agreement with A/S for divergence [ 50].
The alternative calculation method was performed using CONTEST and estimates of variation in amino acid charge and volume revealed 79 sites with signatures of positive selection for at least one of the amino acid properties (Additional file 8: Table S6).
Correlation analyses were used to test for the following associations: (i) population-mean growth rate between the three experimental temperatures, (ii) population-mean growth rate at each temperature and climatic conditions at collection sites, (iii) estimates of genetic variation and population-mean growth rate, and (iv) plasticity estimates and population-mean growth rate.
Each sample was analyzed in duplicate, and a pooled tissue sample was included in each plate to estimate the interassay coefficient of variation and the coefficient of variation, which was determined to be 4.9%.
Then one builds confidence intervals, and computes estimates of variation enabling hypothesis testing.
Accurate estimates of rate variation, and thus accurate inferences regarding the causes of rate variation, depend on accurate estimates of substitution rates.
If true, classical methods ignore this effect and mislead the true estimates of variation.
Using analysis of variance (ANOVA) for several means, we set the statistical power to 0.80 based on estimates of sample variation and effect size obtained in our pilot experiments.
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