Sentence examples for estimates of template from inspiring English sources

The fragment specific estimates of template numbers are consistent with the estimates based on limiting dilution (see Figure 9, rank correlation around p = 0.5 for most of the fragments), but indicated that variant recovery in fragment F5 was sometimes poor, consistent with the difficulties we encountered when amplifying fragment F5.

Using a series of biological scenarios, we demonstrate that this approach can provide an accurate estimate of initial template concentration.

Using this technique, the amount of organic template is consumed in the synthesis (occluded in as-synthesized MCM-41), thus can be estimated (Note: the quantity of template lost during the purification process of the samples is also taken into account).

The relative Ct, the threshold of cycle value, was used to estimate the initial amount of template in reactions.

The relative Ct (threshold cycle value) method was used to estimate the initial amount of template present in the reactions by Applied Biosystems SDS 2.0.

For quantification of transcript abundance, a standard curve was generated using purified rpoD PCR product over a dilution range of known concentrations and rpoD transcript quantity in cDNA samples determined by quantitative real-time PCR was used to estimate the relative amount of template concentrations of the experimental genes.

Here I show that the most common methods of calculating classification images by averaging stimulus noise samples within stimulus-response classes of trials are much more general than has previously been demonstrated, and that they give unbiased estimates of an observer's template for a wide range of decision rules and non-Gaussian stimulus noise distributions.

The conversion factor was generated by first estimating the concentrations estimates of the synthetic Scorecard templates, and secondly, deducing the factor by using linear regression.

The standard PCR conditions were: 94°C for 2 min, followed by 25 cycles at 94°C for 15 s, 58°C for 30 s and 68°C for 5 min. The concentration of amplified DNA was determined by means of Quant-iT dsDNA HS Assay Kit (Invitrogen), and the number of template doublings was estimated.

Through comparison of allele frequencies estimated from pooled DNAs with those obtained from sequencing of individual templates it is apparent that pooling of template DNAs yields relatively accurate allele frequency estimates and a very low rate of false positives.

In 0.5 unit of polymerase there are about six cycles left before the ratio of estimated template and estimated polymerase reach unity.