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Estimates of overlap between these databases have ranged between 8% and 60% [ 11- 14].
These estimates of overlap are likely to be an under-estimate of the true level of overlap as the 'replication' studies in brain tissue have smaller sample sizes and thus lower power than Westra et al. [ 32].
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Although we started from restrictive datasets, our estimates of overlapping protein-coding genes (Table 1) were consistent with previous analyses in human, mouse and Drosophila [ 13, 27, 29- 31].
The genome sequences of both A. gambiae and A. aegypti contain gaps that could affect our estimates of overlapping genes in the genome assemblies, but we find this unlikely based on our observation that the overlapping genes are distributed throughout the genome in each species without any bias to specific chromosomal region of A. gambiae or specific supercontigs of A. aegypti (data not shown).
Together this means that any estimate of overlap based on GI or name will be an underestimate.
Therefore, the results presented in this study are probably a conservative estimate of overlap between studies.
For measures of the temporal distribution of females, we used (1) data on estimates of estrous overlap based on demographic and life history parameters and (2) two proxies for expected overlap based on breeding seasonality.
Our estimates of niche overlap therefore may not represent cases of true sympatry at the within-site level.
Our estimates of PSC overlap give upper bounds.
Most such studies have independently mapped eQTLs in two or more tissues and contrasted the number of eQTLs found in both tissues, arriving at estimates of the overlap between a pair of tissues ranging from 12 to 80%%.
This was the approach taken when comparing resource use by endaemic skinks threatened by invasive shrews (Brown et al. 2014b) and, as expected, removing rare items increased estimates of resource overlap.
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