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Moreover, most of our estimates of duplication time were less than the species divergence time reported by Tamura [ 38].
Another explanation is that the available full genome sequences do not allow for appropriate estimates of duplication rates in these clades.
If the cancer trait has a larger heritability, then a sample size of 400 will provide good estimates of duplication rates.
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For duplications we chose a threshold of 10 consecutive SFP sites with a fold change of greater than 4-fold as a conservative estimate of duplication.
This result holds for all three duplication age groups defined by the age (in Ks units) of the preceding speciation serving as the upper-bound estimate of duplication's age.
Specifically, with the empirical estimates of the duplication rates used above for the linear BDIM, the quadratic model gives the mean family formation times ~1011 yrs.
The authors argued that the majority of such products resulted from single crossovers on the basis that the rate determined in small-pool PCR of ~1.5 × 10-5 wasimilarar to estimates of CMT1A duplication frequencies based on patient prevalence (0.98 6.5 × 10-5) [ 24].
It is thus unlikely that their inclusion altered the pooled risk estimate because of duplication.
Figure 7 shows the phylogenic relationships between the tandemly arrayed genes at the amino acid level, the estimated time of duplication, and the form of selection experienced (using Ka/ Ks).
In the tropomyosin cytoskeletal gene, independent duplication of many different exons has occurred in most bilaterian lineages (Vrhovski et al. 2008; Irimia, Maeso, et al. 2010; Koziol et al. 2011; fig. 1) at a frequency statistically significantly higher than expected even from the highest estimates of intragenic duplications (Gao and Lynch 2009).
The resulting null distributions of duplications, losses and net expansions/contractions of amino acid transporter families at each node were compared to the values inferred by Notung, as it yielded the most conservative estimates of expansions/contractions, duplications and losses in Sternorrhyncha.
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