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The question regarding whether quantitative estimates of bacteria, determined using PCR-based methods, correlate with the number of viable bacteria has been raised before [ 30].
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On the contrary, the combination of various FISH probes targeting the Firmicutes produced a higher estimate of bacteria belonging to this phylum than the clone libraries.
For the t = 24 Jabba mutant embryos, approximately 1/3 of the fields had the high bacterial count (∼180 ± 16.1 bacteria), and the rest typically had 10 30 bacteria per field, leading to an estimate of 840 bacteria per embryo.
For the t = 24 wt embryos, the average maximal field had 21.7 ± 9.2 bacteria, but there was only one such field per embryo, typically with 3 4 other areas with 1 5 bacteria per field, resulting in an estimate of 33 bacteria per embryo.
There are an estimated 600 species of bacteria that normally inhabit the human oral cavity.
The estimated total number of bacteria analysed was 108.
A CFX96 Real-Time system (Bio-Rad, CA, USA) and SYBR® Premix Ex Taq™ II (TaKaRa, Dalian, China) were used to express quantitative PCR (qPCR), to estimate the abundance of bacteria in jejunum and ileum.
These studies typically use sequence variation in a short fragment of ribosomal genes (e.g. 16S rDNA) for estimating the diversity of bacteria in the sample.
By 48 hr, we estimate that thousands of bacteria were present in the Jabba embryos.
Bacterial growth curves were performed by estimating the titers of bacteria in leaves up to 4 dpi [ 13].
Quantitative culture of adherent bacteria on identically prepared screws was performed to estimate the number of bacteria given to the animals.
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