Incorporation of the thymidine analog bromodeoxyuridine (BrdU) was used to estimate cell proliferation in two functional areas of the lens epithelium: the mitotically active germinative zone (GZ) and the less proliferative center zone (CZ).
Further IHC assessment of the nuclear antigen Ki67 was used to estimate cell proliferation.
Wound healing assays have been employed to study cell polarization or tissue matrix remodeling or to estimate cell proliferation and migration rates.
This analysis was performed to estimate cell proliferation during the treatment as well as to trace newly generated cells (Fig 7A).
To estimate cell proliferation rates of tumour and normal stromal cultures, we used staining with Hoechst 33258 to plot cell growth curves at passage 6 of culturing.
To estimate cell proliferation in lung tissues, standard immunohistochemistry with a mouse monoclonal antibody against PCNA (Santa Cruz Biotechnology, Santa Cruz, CA) was performed on the paraffin sections.
To estimate cell proliferation differences between WT and hs:ARF fins, EdU pulse-chase experiments were performed at 2, 4, and 6 dpa.
Cell number was also used to estimate cell proliferation by plating the A10 cells in six-well plastic culture dishes at a density of 1 × 10/well in DMEM with 10% FBS.
While this paper focused on the detailed presentation of the new model, the authors plan to use the presented model in future work to estimate cell proliferation parameters from real data which was out of the scope of this contribution.
To estimate cell proliferation, cultures of T-MPCs and BM-MPCs plated at 1 × 104 cells per square centimeter in 12-well plates in basal medium were analyzed on days 3, 4, 7, 10, 12, and 14, using MTS (methanethiosulfonate) assay according to the protocol of the manufacturer (Promega Corporation, Madison, WI, USA).
