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The molecular mass of the recombinant CAN1 and CAN2 proteins, estimated using Western blot analysis, was approximately 36 kDa and 39 kDa, roughly the same as predicted from the deduced amino acid sequences: 36.1 kDa and 37.3 kDa, respectively.
The concentration of Nox4 in cell lysates was estimated using Western blotting and allowed calculation of a turnover of ∼200 mol of H2O2 mol−1 (mof of Nox4)−1.
The amounts of the mitochondrial respiratory chain complexes (I IV), the complex I regulatory factor AIF, and ERK1/2 and phospho-ERK1/2 were estimated using Western blot analyses.
The expression of endoplasmic reticulum stress markers was estimated using western blot analysis, and the results showed that compared to wild-type motor neurons, there was an increase in the expression of BiP, ATF4 and CHOP in AR100 motor neurons (P < 0.05, t-test, for all markers, wild-type versus AR100).
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DALYs estimated using the Western Australian ' excess' case fatality rate from the WA hospital morbidity database after 28 days following stroke and disability weights for stroke [ 14].
Densitometry of Western blots was estimated using ImageJ software.
Densitometry of Western blots was estimated using ImageJ software (NIH).
The integral optical density (IOD) of the Western blot band was estimated using ImageJ.
Cancer incident cases and deaths were estimated using age-specific rates, which were stratified by area, region (eastern, middle, western), cancer type, sex, and age.
LD was estimated using 1000 Genome Centre d'Etude du Polymorphisme (CEU) (Utah residents with northern and western European ancestry) data from SNAP (Broad Institute, Cambridge, MA, USA) [ 11].
Protein concentrations were estimated using BCA reagent and equal amount of proteins were subjected to native PAGE followed by western blotting.
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