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The insert sizes of the 19 sugarcane BACs with insert were estimated using pulse field gel electrophoresis, ranging from 40 - 165 kb with an average of 97.6 Kb (Table 1).
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The precise timing of diapause termination in populations of W. smithii has been estimated using "pulse-chase" experiments [28].
It should be remembered, however, that although this number is relatively large compared to other free-living protists (e.g., 27,000 genes in the ciliate Tetrahymena thermophila [24] and 12,000 genes in the diatom Phaeodactylum tricornutum [25]), it does not account for the massive amount of nuclear DNA (ca. 150 Gb, estimated using pulse-field gel electrophoresis) in haploid Alexandrium cells.
The new model structure, termed as the Non-linear ECM (NL-ECM), gives a lower Root Mean Square (RMS) and peak error when compared to an ECM estimated using a pulse data set.
Also, the speed of the current degradation is estimated using the number of pulse cycles.
Based on angle-Doppler estimates, the range is then estimated using frequency-varying received pulses.
General properties of the postsynaptic response (peak amplitude, 20 80% rise time, and 100 37% decay time, paired-pulse ratio) were estimated using the Clampfit program (Molecular Devices).
59 Pulse pressure will be estimated using the mean values of the second and third measurements.
Pump pulse spot diameters of 250 360 μm were estimated using a micrometer stage and a razor blade; the broadband probe pulses were focused to ∼50 μm.
LV stroke volume can be estimated using a beat-to-beat based, algorithmic analysis of arterial pulse pressure [ 24].
The ASE intensity has been estimated using measured dependence of amplification time with respect to different seed pulse energies.
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