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On the euglycemic day, renal hemodynamic function [glomerular filtration rate (GFR) and ERPF] were estimated using inulin and p-aminohippuric (PAH) clearance techniques.
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1/S' was estimated using BlastPhen.
Nucleotide diversity was estimated using DnaSp 4.10 [38].
Effects were estimated using conditional logistic regression.
There was also a trend toward a similar association of GFR estimated using MDRD with the 2677T (8.6 ± 5.8 P = 0.14) and the 3435T (10.1 ± 5.3, P = 0.058) alleles in analysis of MDRD in the 119 subjects for whom inulin clearance values were missing.
A subsequent paper by Oppelt and Rall [ 4] exploited this concept to make some of the first estimates of volume fraction and effective diffusion coefficients, primarily using inulin and sucrose in several brain regions and animal species, including: "live dog, dead dog, live cat, dead cat, live green monkey, dead green monkey, live rhesus monkey, dead rhesus monkey".
Indeed, correct estimation of glomerular filtration rates implies its determination by using inulin or iohexol clearance, and radionucleotide.
To further monitor the integrity of the PGEC barrier, we also measured the paracellular passage of the extracellular marker inulin by determination of the permeability coefficient as evaluated by a diffusion assay using inulin as described previously [19].
GFR and RBF were measured using inulin and PAH, respectively, according to established methods.
Moreover, accurate GFR measurements using inulin or iothalamate infusions are undoable for large scale study populations.
In the first two studies, GFR was measured using inulin clearance.
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