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To ensure equal protein content on each lane after electro blotting membranes were incubated with Ponceau red (top row), and protein amounts were estimated using densitometry.
Coomassie-stained protein bands were ultimately scanned, and the intensity of each recombinant protein band was estimated using densitometry (Duoscan T1200, Bio-Rad, Hercules, CA).
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Portions of the ligation products were estimated using ImageJ (http://imagej.nih.gov/ij/) densitometry analysis with two technical replicates.
Films were scanned and densitometry values estimated using ImageJ (NIH) software.
The genome sizes of five naid species were estimated using the Feulgen image analysis densitometry method [ 90].
Densitometry of Western blots was estimated using ImageJ software (NIH).
Densitometry of Western blots was estimated using ImageJ software.
The dimer:monomer ratios were estimated using ImageJ software (NIH, Bethesda, USA) to analyse densitometry of photographs of the gradients, as described in [22].
Using densitometry, we estimated that mAbs KIM127 and 38, but not mAb 24 or control mAb, immunoprecipitated 0.45±0.1% of total α-actinin-1 (n=3).
MMP-2 contents were estimated by gelatin zymography (Mirastschijski et al., 2002) relative to MMP-2 standard run in parallel using densitometry (ImageJ).
Quantification of immunoreactive bands was performed using densitometry.
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