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Biofilms were generated from strains MG1655 and PHL628 (a) or MC4100 and PHL644 (b) with and without pSTB7 using the spin-down method, matured for 7 days in M63 medium and biomass was estimated using crystal violet staining.
Fed state and 6 h fasted blood samples were collected by the retro-orbital puncture and insulin levels were estimated using Crystal chem kit (ELISA).
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Distributions of activated deformation modes under the indentation tests were estimated using a crystal plasticity finite element method.
Random coincidences were estimated using single crystal event rates.
The lattice constants and unit cell volumes of the Sr1−x Ba x W1−y Ta y O4−(1/2)y crystals estimated using the XRD results are listed in Table 1.
The average crystal sizes were estimated using the Scherrer equation.
The accuracy of the ensemble was estimated using the RMSD of the cluster center of the largest cluster to the gold-standard crystal structure.
1/S' was estimated using BlastPhen.
Confidence intervals were estimated using normal approximation.
RNA concentration was estimated using nanodrop spectrophotometer.
Divergence times were estimated using Multidivtime [52] [54].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com