The pI and molecular weight were estimated using the Compute pI/Mw tool from ExPASy (http://web.expasy.org/compute_pi).org/compute_pi
The isoelectric points and molecular weights were estimated using the Compute pI/Mw tool from ExPASy (http://web.expasy.org/compute_pi).org/compute_pi
Primer sets used are listed in Additional file 4. The isoelectric point and molecular mass predictions were estimated using the compute pI/Mw tool (http://expasy.org/tools/pi_tool.html).html
The haplotype frequencies were estimated using GENECOUNTING, which computes maximum-likelihood estimates of haplotype frequencies from unknown phase data by utilising an expectation-maximisation algorithm.
Significance of over-representation is estimated using binomial distribution by computing a nominal p-value.
We then plot the best fitting exponential distribution with parameter λ estimated using the Maximum Likelihood Estimator, computed as the inverse of the sample mean (λ = 1/μ = 0.13).
The per-pixel non-uniformity, a i, can be estimated using a flat field image computed as the average over a large sequence of non-saturated images.
Variance components were estimated using the anova method to compute a coefficient of determination R for each ecotype.
The estimated joint statistics were computed using Σ estimated using Adapt-Mix.
Albert C. Lardo, Amir Ali Rahsepar, Jung Hee Seo, Parastou Eslami, Frederick Korley, Satoru Kishi, Thura Abd, Rajat Mittal, Richard T. George, "Estimating Coronary Blood Flow using Computed Tomography Transluminal Attenuation Flow Encoding (TAFE): Formulation, Preclinical Validation and Clinical Feasibility", Journal of Cardiovascular Computed Tomography, ISSN 1934-5925.
