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SOD activity was determined by the method of Sun et al. Plasma concentration of NO was estimated using colorimetric assay.
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Tannin content of each substrate was estimated using the colorimetric method of Hagerman and Butler (1978).
Iron content was estimated using a colorimetric ferrozine assay [19].
Total phenolics content was estimated using a colorimetric assay [ 16].
The amount of TSP was estimated using the Bradford colorimetric assay as specified by the manufacturer (Bio-Rad, Hercules, CA, USA).
Caspase 3 activity was estimated using a caspase 3 colorimetric assay kit (Sigma-Aldrich).
Caspase 3 activity was estimated using the caspase 3 colorimetric assay kit, which detects enzyme activity based on the cleavage of Asp-Glu-Val-Asp (DEVD -pNA (R&DEVD -pNA Inc., Minneapolis, MN, USA).
Total terpenoid content was estimated using the method of [25], total tannin content was determined using the procedure [26], total phenolic content was estimated by Folin- Ciocalteau method [27] and total flavonoid content was determined by the aluminium chloride colorimetric method [28].
1/S' was estimated using BlastPhen.
Finally, total phenolic and flavonoid contents were estimated in the extracts using colorimetric assays.
The detection limit for Hg2+ ions using colorimetric sensors based on the ruthenium complexes was estimated to be 20 ppb [21].
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