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Thus, photoreceptor quantum catch was estimated using absorbance spectra.
The protein concentration of purified recombinant enzyme was estimated using absorbance at 280 nm and the computed molar extinction coefficient of the protein sequence.
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The quantum catch of photoreceptors should ideally be estimated using absorbtance rather than absorbance spectra, with the former depending on the transverse specific density of pigments and the outer segment length of photoreceptors.
The latter were estimated using ultraviolet absorbance (UVPM), fluorescence (FPM) and solanesol (SolPM) measurements.
The drug release kinetics of GNP-Chl conjugates revealed the release of chloroquine at an acidic pH, which was quantitatively estimated using optical absorbance spectroscopy.
RNA concentration and purity were estimated using spectrophotometry (absorbance at 230, 260 and 280 nm).
Protein concentration was estimated using UV absorbance 280 nm and 260 nm and determined by the Bradford assay [ 7] using albumin as the protein standard.
The concentration for ELPs was estimated using their absorbance at 280 nm according to molecular extinction coefficients of 1280 and 3760 M 1 cm–1 for A192/A192-RGD and A192-VCN/VCN, respectively.
These values are higher than the semiconductor nanoparticle sizes estimated using the UV vis absorbance curves and TEM analysis (2.1 to 2.5 nm).
The nucleic acid concentrations were measured with a Qubit 2.0 fluorometer (Life Technologies), and the purity of the total RNA was estimated using the 260/280 nm absorbance ratio on a NanoDrop™ 2000 (Thermo Scientific) machine.
Tyrosine released was estimated using Folin Ciocalteau's reagent and absorbance was taken at 670 nm.
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