The absolute limit of detection (LOD), defined as the lowest concentration of wt sequence detectable in all the nine replicates, was estimated to be about 14 genome copies, corresponding approximately to 0.01% wt, while the same target was detected in only six replicates when 2.8 copies were present (Table 2).
Whether the money, estimated at $50 million to $100 million to replicate or replace vintage architectural and structural details, will be forthcoming could depend on the insurance settlement.
To estimate our power to replicate the published association, we assumed a difference per Trp allele of 1·04%, as reported by Habib et al.
A recent study estimated the required sample size to replicate an association finding with different amounts of between-study heterogeneity [28].
Finally, we estimated the average time taken to replicate a chromosome under various conditions by determining growth rates and oriC/ ter ratios [ 26].
A highly-detailed traffic model was built in AIMSUN 8.1.2 model (Advanced Interactive Microscopic Simulator for Urban and Non-Urban Networks) for this case study to replicate and estimate traffic conditions and its related environmental and operational effects.
Fourth, this whole process is repeated 1,000 times, each leading to replicate estimates.
By using the ULSAM cohort, although with a lower number of fractures rendering less precise estimates, we were able to replicate the findings from the SMC, which was a further strength of our study.
This was achieved by resampling with replacement from the original replicate fitness estimates to generate 10,000 sets of 24 versus six pseudo-populations (or 12 versus 6 pseudo-populations for the analysis of fitness trajectories).
Monte Carlo simulations are carried out to replicate datasets, estimate prediction model parameters and validate them using Lin's concordance measure.
