The level was estimated to be approx. four-fold higher than in the control transformant.
Reduction rates in faecal egg counts are estimated to be approx. 2% annually [ 42] but the reduction in anthelmintic treatment frequency remains to been demonstrated.
Agricultural Pi deficiency is alleviated by the massive application of Pi fertilizers, estimated to be approx. 40 million metric tons per year worldwide [ 8].
The decrease in BTPC's I50(malate) from ~6.5 to 1.5 mM caused by the S425D mutation is physiologically relevant because the concentration of malate within the endosperm of mid-cotyledon (stage V) developing COS has been estimated to be approx. 5 mM [ 44].
On the other hand, only a single revertant was obtained in one of the three replicate experiments with the strain carrying an A7 out of frame kanR fusion, indicating a low A7 → A6 reversion frequency (which can be estimated to be approx. 3.9 × 10-8; see Additional File 3).
The energy of the surface states was estimated to be approx. 0.6 eV below the conduction band, and the rate constant kirr for the irreversible transitions has been determined to be 10−1 cm s−1.
The concentration of the zymogen appears to be higher than that of mature caspase-3 [ 38], although HeLa cells are estimated to contain approx. 120 nM procaspase-3 [ 28].
Atlantic salmon and rainbow trout are estimated to have split approx. 20 mya [ 25].
In addition, nitrogen and phosphorous loads to Køge Bay are estimated to be reduced by approx. 63 t yr.− 1 and 9 t yr.− 1, respectively, contributing to the achievement of more than 70% of the nutrient reduction target set for Køge Bay in the first WFD river basin management plan.
Additionally, the method of chromatographic separation allowed for the detection of BMPEA in the No-shotgun supplement, in which it was estimated to be present at approx. 770 μg/g (16 mg per serving; our unpublished results).
Upper-body subcutaneous fat is relatively resistant to insulin suppression of lipolysis and is estimated to be the source of approx. 60% of circulating NEFAs (non-esterified fatty acids).
