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We estimated the expression level of each contig by counting 454 reads mapped back to the BT_transcriptome_v2 dataset.
For each mapped RNA-seq library, we then estimated the expression level of each transcript with its respective error, for each allele in a Cast/EiJ×C57Bl/6J diploid transcriptome using MMSEQ (Turro et al., 2011).
We finally estimated the expression level of the nearest neighbor TAR by computing the average intensities of the virtual tiles and compared this value with the expression level measured from the actual probes within the TAR (determined by averaging their PM-MM values).
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We exposed HeLa cells to CHX under hypoxic conditions in the presence or absence of TPZ at different time points and estimated the expression levels of HIF-1α.
Hence, we estimated the expression levels of HSP47 following incubation with the cocktail.
We estimated the expression levels of NFκB and IκBα using western blotting.
Following that, we estimated the expression levels with their respective uncertainties of each transcript in our C57Bl/6J, Cast/EiJ diploid transcriptome using MMSEQ (Turro et al., 2011).
When we estimated the expression levels after infection at each separate time point, similar results were observed (Additional file 1: Table S5).
The positive correlation on a linear regression model between qRT-PCR and RPK values and the high R-squared value demonstrated that RNA-Seq data properly estimated the expression levels of the selected genes.
Finally, we estimated the expression levels of the proteins under study in 16 cultures of lung tumour stromal cells and in 13 cultures of normal lung stromal cells (Supplementary Figure 3).
Given the high-sequence homology between different MT isoforms, none of previous studies have discriminated and estimated the expression levels among different MT family members or at least among MT I/II.
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