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The proposed impulse model incorporates the duty ratio and input frequency of the triangular waveform of counter mass relative displacement as the system input and outputs the estimated step size and the behavior of the stepping motion.
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The Qdot assay routinely estimates step-size from low duty cycle muscle myosins using a standard research microscope setup.
Optimizing conditions for estimating step-size follows the previously established guidelines usually requiring 2 or 3 measurement analysis cycles to pinpoint optimal parameters for exciting light intensity, Δ t, camera exposure time, and bulk concentration of motor protein.
In this paper, backtracking line search, which is also known as the Armijo rule [36], is used to estimate the step size.
(2004) estimated the kinetic step size of unwinding by T7 helicase to be ∼10 bp.
However, the kinetic step size estimated from ensemble measurements can be inflated if there exists significant heterogeneity in the reaction rate among individual molecules (Park et al., 2010).
In our case, the step size estimated by diving 32 nt by 26 steps is 1.2 nt per step, and considering the likelihood of overestimation due to errors, the true step size must be 1 nt.
This estimate determined the step size and range of values in the Method of Constant Stimuli, used throughout the remainder of the experiment to estimate positional thresholds.
Thus one can pool the values to get a better estimate of the step size.
With imprecise error estimates, the step-size adjustment has to be conservative to preserve accuracy, or it risks producing an imprecise result.
Provides a strategy for estimating the critical time step size using CAG elements, nodal integration or SFEM.
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