[125I]Motilin binding was used to estimate receptor expression level for the stable cell line, and titration of a membrane preparation indicated a Kd value of 0.8 nM.
Granulosa cells in monolayer culture exhibit heterogeneous receptor induction, indicating that normalized [125I]iodo-hCG binding data cannot be used to estimate receptor concentration per cell.
Another possible way for estimating receptor occupancy is to estimate a relationship between blocking dose (or plasma level) and VT for each region [31].
Based on saturation binding studies [12], which incorporate NLX's affinity to MOR, we estimate receptor occupancy of 10 ng/kg NLX as no more than 1%.
To estimate receptor surface expression, we used an indirect cellular ELISA [ 39].
We used the IC50 values shown in Table 1 (from the competitive receptor-binding assay for nuclear ERα, ERβ, and ERRγ) to estimate receptor selectivity ratios for BPA and bisphenol AF (Table 2).
In addition, Rappel and Levine [ 18] used Monte Carlo simulations to estimate receptor-ligand binding noise, a second important noise source, and then applied this noise input to two chemotaxis models.
Expression levels of the remaining wild-type receptor genes were examined to estimate the receptor amount in each receptor mutant, and to evaluate if effects of ers1 mutations on the ethylene response phenotype were due to receptor functional compensation.
Considering all gratings and the setup geometry, a measured value of 0.22 mGy corresponds to an estimated image receptor dose of about 25 μGy for 1-s exposure time.
The reason that Model 2 led to a much larger difference in the PFC activity between patients and controls is that the experimentally estimated D2 receptor binding is higher than that in Model 1.
