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To overcome this, a study was conducted to design a specific quantitative real-time PCR (qPCR) assay using TaqMan®MGB (minor groove binding) probe for its identification and estimated quantification in contaminated MWFs.
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In estimating quantification ratios, peptides where the total ion counts of iTRAQ peaks were below a threshold of 40 were excluded.
The data was analysed using Sequence Detection Software SDSS) V.1.4 with manual baseline/threshold settings to estimate quantification cycle.
To estimate quantification of the additional predictive ability of adding falls to the clinical risk factor model we looked at the AIC which shows the biggest improvement in the model with the addition of falls, suggesting it is a stronger risk factor than age, height or weight.
From our data, we estimated a quantification threshold for temperature variation of 1.2 °C.
The yield of the DTPA coupling reaction was estimated by quantification of the protein gel electrophoresis, and dividing the band with DTPA derivatives by the sum of all bands (Figure 5b).
Total particle concentration of the viral stocks was estimated by quantification of the p24 capsid protein using RETRO-TEK HIV-1 p24 Antigen ELISA kit (ZeptoMetrixCorporation, Buffalo, NY USA) according to the manufacturer's instructions.
Given the inverse relationship between Tfr1 protein and hepatocyte iron content, this Tfr1 profile reflects the iron content estimated by quantification of the Perls' Prussian Blue staining (Figure 1B).
At the time of parasite perfusion, hepatic iron stores were estimated by quantification of Perls' Prussian Blue staining for non-heme iron within liver sections (figure 1A and B).
Using the estimated variance, quantification of the m/ z distribution across multiple scans was achieved by a nonlinear fitting approach.
Mos1 cleavage activity is inhibited by 10 to 500 μM Raltegravir with an IC50 of 60 70 μM as estimated from quantification of the % of supercoiled plasmid in each reaction.
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