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The mean γH2AX and activated ATM IF intensity was estimated per sample.
Diversity indices and rarefaction curves were estimated per sample using Mothur [ 28] based on OTUs generated by SILVA-ngs.
This model is estimated per sample, as the phenomenon is modulated by TUF, the concentration of the DNA input, and possibly other factors.
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Census and effective population size estimated per sampling site were not correlated (effective size versus census size: F1,7 = 0.17, adjusted R² = −0.12, P = 0.70) (Table S3).
The maximum likelihood estimates of A appear to be biased slightly upwards, but this is difficult to know with certainty due to there being only ten estimates per sample size.
Estimated per person.
Each variable was estimated per household.
To estimate CFUs per sampled surface area, the number of CFUs per plate was multiplied by 20 (2 mL extract solution divided by 0.1 mL plated solution) and divided by the recorded surface area in square centimeters.
To estimate CFU per sampled surface area, the reported CFU/g were multiplied by the reported weight of the sock contents and divided by the recorded surface area in square centimeters.
However, both direct tests exclude the need for prior culture to isolate M. tuberculosis before performing DST, which explains the lower cost of direct NRA compared to the indirect LJPM (estimated costs $4.12 per sample).
Regional species richness was estimated by species per sample accumulation curves plotted against abundance, with order of sample input randomised 50 times to remove distortions due to the stations lying along a productivity gradient; productivity is known to affect deep-sea nematode diversity [ 5, 16].
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