Sentence examples for estimated in duplicate from inspiring English sources
Salivary concentrations of cortisol were estimated in duplicate using commercial radioimmunoassay kits (Siemens Medical Solutions Diagnostics, Los Angeles, CA).
Concentrations of cortisol in the aqueous component were estimated in duplicate using commercial coated tube RIA kits (Siemens Medical Solutions Diagnostics, Los Angeles, CA).
If spermatozoa were present, their numbers were estimated (in duplicate) using an improved Neubauer haemocytometer and gross morphology was noted by microscopic examination (×200).
Concentrations of tumour necrosis factor-alpha (TNFα), interleukin-6 (IL-6), and IL-8 in sera were estimated in duplicate by an enzyme immunoabsorbent assay (Diaclone, Paris, France).
suPAR was estimated in duplicate by an enzyme-linked immunosorbent assay (suPARnostic™; ViroGates, Lyngby, Denmark) in accordance with the instructions of the manufacturer; the lower detection limit was 1.1 ng/mL.
IgM was estimated in duplicate by an enzyme-linked immunosorbent assay (e-Bioscience Inc., San Diego, CA, USA) following the manufacturer's instructions; the lower detection limit was 20 ng/ml.
Also, the Cq of samples to be quantified should be estimated in duplicates or more.
Standard curves of SYBR-Green qPCR for M, HA, and NA genes were established on 10-fold serial dilutions of reverse transcribed cDNA of reference viral RNA from 5 pg/mL to 1 ag/mL to estimate in duplicate the system's liner range, efficiency, and detection limitation.
The values given in the Table are the mean ± standard deviation of the kinetic parameters estimated in two duplicate saturation experiments performed with enzymes from two different purification batches.
The ordinary-, penandzed-, and bootstrap t-test, least squares and best linear unbiased prediction were compared for their false discovery rates (FDR), i.e. the fraction of falsely discovered genes, which was empirically estimated in a duplicate of the data set.
Concentrations of IL-1β, IL-6 and ΤNFα were estimated in supernatants in duplicate by an enzyme immunoassay (R&D Systems, Minneapolis, MN, USA).
