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IDBA-Tran also provides an estimated expression level for each transcript by aligning reads to the transcript.
For all probesets we estimated expression level in each cell-line using the Plier Sketch algorithm and estimated detection above background probabilities using DABG [ 53].
These data are presented in a table that contains the name, total length, and estimated expression level for 17,082 different genes of C. elegans.
While the expression level of the fliT, estimated by the sample mean at -0.90, ranked only 65th, the estimated expression level of the gene after borrowing information from two other genes in the operon was -3.25, which ranked 19th.
Statistically significant differentially expressed genes were binned into up- and down-regulated classes based on an estimated expression level under the SAS model.
To evaluate the performance of the proposed method in detecting DE genes, the estimated expression level of each gene was used to rank the genes, and the highly ranked genes were identified as DE genes.
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Therefore the estimated expression levels of weakly expressed genes have greater variability than highly expressed genes.
We estimated expression levels using HTseq v0.6.1 to count reads, and then calculated reads per kilobase of transcript per million mapped reads (RPKM).
Specifically, we estimated expression levels and promoter methylation rate for 13,847 genes.
For USC vs. EAC-G1, 7 of 9 genes showed at least 1.5-fold changes in qRT-PCR estimated expression levels and were concordant with the microarray data, yielding a validation rate of 7/9 (Figure 5c d).
Log-log scatter plot of the estimated expression levels as nRPK.
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