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We estimated cell viability for flat sheets, and performed histological examination, analysis of extracellular matrix (ECM) deposition and mechanical tests on tubular constructs.
Estimated cell viability by trypan blue exclusion was routinely higher than 95%.
Estimates of Cu accumulated per cell was obtained by standardising total Cu accumulated against estimated cell viability (OD).
We treated MSH2 deficient and proficient cells with methotrexate alone, or in combination with 1 µM selenomethionine and then estimated cell viability and 8-OHdG levels.
In addition, in our study, AsPC-1 cells were pretreated with 3-MA (inhibitor of PI3K III) in autophagy signalling) at increasing concentrations from 0 to 1 m M for 30 min before treatment with IC50 of PM-17 for 1 24 h, and estimated cell viability using the MTT assay.
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Plates were incubated for 24 h at 37 °C and colony forming units were enumerated to estimate cell viability (Velliou et al. 2011).
An ATP assay was used to estimate cell viability.
For cytotoxicity analysis, the CytoTox-ONE™ Homogeneous Membrane Integrity Assay Kit (Promega, Madison, WI) was used to estimate cell viability.
To estimate cell viability after dye loading, we performed a live/dead assay in cells after 10 days of VPA differentiation.
The MTT conversion assay (Chemicon International Inc., Temecula, CA, USA) was used to estimate cell viability (Tomita et al, 2003).
Propidium iodide (BD Biosciences) was used to estimate cell viability and FITC- and PE- conjugated mouse IgG1 (BD Biosciences) were used as isotype controls.
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