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Let φ g denote the estimated phase angle of gene and let T (in minutes) denote the estimated cell cycle period of a particular experiment [ 52].
In comparison, the proliferating proportion of the SVZ population of cells have an estimated cell cycle length of ∼12.7 h and an S phase of ∼4.2 h [64].
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In order to test the validity of using antibodies to estimate cell cycle phase in situ, double labelling experiments were performed in ovarian serous borderline tumours and serous cystadenocarcinomas (n=6 in each group) as previously described (Scott et al, 2003).
We have also demonstrated the potential for antibodies raised against cyclins D1, A, B1 and phosphorylated histone H3 to estimate cell cycle phase distribution by immunohistochemistry (Scott et al, 2003).
The remainder of the subsections that follow describe the new sampling model we developed in this work to estimate cell cycle progression parameters from any number or combination of binary-valued markers.
Nuclear DNA at sub G1 phase in normal and extract treated HL-60 cancer cell lines was estimated by cell cycle analysis using flow cytometer.
Analysis of confocal images of root cortical cell profiles from the MZ through the EZ was then used to estimate cell production rates and cell cycle lengths in this cell layer ([ 45], and see Methods).
To estimate the cell cycle duration of type A undifferentiated spermatogonia (Aund* and Aund), 10 males were exposed to BrdU (Sigma-Aldrich, St . Louis MO, USA, http://www.sigmaaldrich.com) dissolved in water (4 mg/ml) for approximately 4 and 10 h.
This strategy estimates cell ploidy based on DNA content (which correlates to the intensity of fluorescence) and the stage of cells in the cell cycle.
This finding, together with the estimated very long cell cycle length in lampreys [13] may partly explain the especially slow growth of its brain.
Cells were analysed by FACS, and the proportion in G0/G1, S and G2/M phases was estimated using Modfit cell cycle analysis programme.
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