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This opens up the potential for total soil carbon levels to be estimated by the quantification of easily measured ecosystem properties.
The main exo-acting glycosidases activities were estimated by the quantification of pNP-α-L-arabinofuranoside (pAra), pNP-α-D-galactopyranoside (pGal), pNP-β-D-xylopyranoside (pXyl) and pNP-β-D-mannopyranoside (pMan).
A relative quantification assay of HIV-1 proviral DNA in purified CD4+ cells was developed on the LightCycler® real-time PCR instrument expressed per 106 cellscells as derived from estimated by the quantification of the β-globin gene.
In the present study, a relative quantification assay of HIV-1 proviral DNA by LightCycler® real-time PCR based on SYBR Green I detection was developed in comparison to the number of purified CD4+ cells as estimated by the quantification of the β-globin gene.
Cellulose degradation was estimated by the quantification of endo-glucanase (carboxy-methyl cellulose, CMC), Avicelase (Avicel, AVI), FPase (Filter paper, FP), cellobiohydrolase (pNP-β-D-cellobioside, pCel and pNP-β-D-lactobioside, pLac) and β-glucosidase (pNP-β-D-glucopyranoside, pGlc) activities.
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The amount of target mRNA in samples was estimated by the 2CT relative quantification method using GAPDH, m36B4 or Hprt1 for normalization.
The stained root samples were examined at 45 100× magnification and quantification of root colonization by AMF was estimated by the gridline-intersect method [51].
For protein quantification the cells were lysed by a CHAPS-based lysis buffer and the protein was estimated by the DC Protein Assay kit from BIO-RAD according to manufacturer's protocol.
The yield of the DTPA coupling reaction was estimated by quantification of the protein gel electrophoresis, and dividing the band with DTPA derivatives by the sum of all bands (Figure 5b).
Given the inverse relationship between Tfr1 protein and hepatocyte iron content, this Tfr1 profile reflects the iron content estimated by quantification of the Perls' Prussian Blue staining (Figure 1B).
Total particle concentration of the viral stocks was estimated by quantification of the p24 capsid protein using RETRO-TEK HIV-1 p24 Antigen ELISA kit (ZeptoMetrixCorporation, Buffalo, NY USA) according to the manufacturer's instructions.
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