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The density of the composite's surface amino groups as estimated by surface imine formation procedure [8] was 0.18 molecules/nm2.
Dispersion curves at these sites are calculated using the F-K method (Capon, 1969) for the vertical component; S-wave velocity structures for the Puli area are then estimated by surface wave inversion (Herrmann, 1991).
The lateral variation in phase velocities estimated by surface wave analysis mainly reflects lithological differences (most sensitive to differences in S-wave velocities) (e.g., Fig. 3a c and MBMTL; Fig. 7d).
It was biologically active based on its interaction with PAI-1 estimated by surface plasmon resonance (SPR) experiments, gel-filtration chromatography, and isothermal titration calorimetry (see Results).
Moreover, we determined that such interaction is mediated by two conserved basic motifs with a dissociation constant (Kd) of 0.6 µM as estimated by surface plasmon resonance (SPR) analysis.
Serum SIRT1, SIRT2, and SIRT3 were estimated by surface plasmon resonance (SPR) and Western blot.
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The Brunauer Emmett Teller (BET) surface area was estimated by a surface area apparatus TriStar-30000, Micromeritics).
The Brunauer-Emmett-Teller (BET) specific surface area was estimated by the surface area apparatus TriStar-30000, Micromeritics).
We compared the amount of EphA4 expressed on the cell surface; this was estimated by cell surface biotinylation, in NIH3T3 cells transformed with EphA4-HA, with or without the exogenous expression of Meltrin β (Fig. 5A).
Then, the optimal operating conditions were estimated by response surface methodology.
Forage ET was also estimated by a surface renewal (SR) method in large pastures of TWG and CWR irrigated with saline DW.
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