Figure 2, C and D, respectively show the results for Cyr61 interaction with immobilized monomeric or multimeric VTNC estimated by solid-phase binding assays.
Urinary albumin excretion rate (UAER) was estimated by solid-phase competitive chemiluminescent enzyme immunoassay (sensitivity of 0.5 mcg ⁄mL; Immulite 1000 Systems; DPC Medlab, Los Angeles, CA, USA) with intra- and inter-assay variation coefficients of 4.4 and 6.1%, respectively.
The binding affinity estimated by solid-phase assay with recombinant protein forms might not necessarily be the same as the affinity to the native structure forms on the cell surface; thus, the equivalency in anti-CD20 efficacy was observed.
( {sigma}_n^{prime } ) was estimated by considering solid density, porosity, and depth of the fault plane (z) under the assumption of hydrostatic pore fluid pressure: {sigma}_n^{prime }=g{displaystyle underset{0}{overset{z}{int }}{rho}_{ z)}dz} (6).
These were assigned the following attributes: (i) charge; (ii) normal vectors that denote the surface direction and ring plane orientation (for aromatic rings); (iii) surface patch size and curvature (estimated by the solid angle shape function [ 52]); Figure 1A presents examples of a representation of amino acids by pseudocenters.
Total dissolved solids (TDS) were estimated by weighing the solid residue obtained by evaporation of a measured volume of water samples to dryness (Chopra and Kanwar 1980).
Trends in 5-, 15-, 15-, and 20-year survival rates of colon cancer patients as estimated by cohort analysis (solid black lines) and period analysis (solid grey lines) are shown in Figure 1.
Such a disagreement between the experimental and calculated values was discussed in relation to the morphology estimated by high-resolution solid state 13C NMR spectroscopy.
The 95% confidence intervals are of the forest clearing estimated by remote sensing (solid red line, 1990-2000).
After infection with S. citri, spiroplasma attachment to and internalization into Ciha-1 cells were estimated by counting colonies on solid SP4 medium as described previously [16].
We measured body weight weekly from weeks 4 to 14. Food intake was estimated by the weight of solid pellets placed on grids on top of the poly-propylene cages, which was measured weekly from weeks 8 to 14.
