Sentence examples for estimated by sequencing from inspiring English sources

The sequence diversity of the library was estimated by sequencing 15 clones, translating each sequence, and comparing the observed frequency of each amino acid with that expected from the randomization scheme used to construct the library.

The correlation between marker allele frequencies determined by sequencing and genotyping was ∼0.83 (considering only the 212 unrelated samples from the Aquitaine G0 breeding population) (Figure S1), showing that allelic frequencies estimated by genotyping were generally in the range of those estimated by sequencing.

The effect of selection on maize diversity has been estimated by sequencing 278 temperate maize inbred lines from different stages of breeding history.

The intensity of the MSP bands (figure 3A) was compared with the methylated CpG content that was estimated by sequencing the common PCR DNA (figure 3B).

Note that allele frequencies estimated by sequencing pools of PCR-amplified DNA may be biased, as the PCR may preferentially amplify one allele compared to the other (Sham et al., 2002).

The correlation between allele frequency estimated by sequencing and genotyping was calculated over 310 observations (Additional file 3) by randomly selecting the major or minor allele.

We found that the apparent loss of gene representation was largely (77%) associated to the high frequency of non-annotated genes (i.e. function unknown or estimated by sequence homology).

Frequencies of re-infection and persistence of HRV-C among subjects who contributed more than one positive sample over the study period were estimated by sequence comparisons in the VP4/VP2-encoding region.

Genetic differentiation between populations was estimated by sequence-based F statistics (Fst) according to Hudson et al. [ 41].

Although the total number of sequences differed among the four sequencing experiments (see Additional file 1: Table S2), gene expression levels estimated by sequence coverage showed high positive correlation between both the biological and the technical replicates (see Additional file 1: Figure S2).

The methylation status of the PXR promoter sequence was also estimated by bisulfite sequencing on at least 7 individual DNA strands after subcloning of PCR products into the pCR4-TOPO vector using the TOPO TA Cloning Kit for Sequencing (Invitrogen).