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The yield of the DTPA coupling reaction was estimated by quantification of the protein gel electrophoresis, and dividing the band with DTPA derivatives by the sum of all bands (Figure 5b).
Total particle concentration of the viral stocks was estimated by quantification of the p24 capsid protein using RETRO-TEK HIV-1 p24 Antigen ELISA kit (ZeptoMetrixCorporation, Buffalo, NY USA) according to the manufacturer's instructions.
Given the inverse relationship between Tfr1 protein and hepatocyte iron content, this Tfr1 profile reflects the iron content estimated by quantification of the Perls' Prussian Blue staining (Figure 1B).
At the time of parasite perfusion, hepatic iron stores were estimated by quantification of Perls' Prussian Blue staining for non-heme iron within liver sections (figure 1A and B).
The conjugation yields of 2K, 5K, and 20K GSBP-PEG were estimated by quantification of the band intensities taking into account the dimeric form of GST, and were calculated to be 52%, 56%, and 76%, respectively (Table 1).
At days 4, 7 and 10 representative samples were fixed in 2% paraformaldehyde solution and B16 F10 proliferation was estimated by quantification of crystal violet staining of the cells with a microplate reader (Gillies et al, 1986).
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This opens up the potential for total soil carbon levels to be estimated by the quantification of easily measured ecosystem properties.
Intensity of immunoreactive bands was estimated by densitometric quantification using the Gel analyzer v1.0 software.
Forty eight hours after transfection, phosphorylated NF-κB, detected at a size of 65 kDa (Figure 2B), was increased 3.7-fold, as estimated by densitometry quantification (p = 0.01) in human AM transfected with CFTR-siRNA (Figure 2C).
For isolated phosphoproteins, the relative protein abundance is estimated by combining quantification of all its detected constituent peptides.
A relative quantification assay of HIV-1 proviral DNA in purified CD4+ cells was developed on the LightCycler® real-time PCR instrument expressed per 106 cellscells as derived from estimated by the quantification of the β-globin gene.
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